NTGP First Year Dissemination Presentation
Nepal Tiger Genome Project first year finding dissemination was presented to stakeholders on April 16 2012 at the Department of National Park and Wildlife Conservation (DNPWC), Kathmandu. Representatives from WWF Nepal, NTNC, DNPWC, MoFSC and USAID Nepal were present. This program was facilitated by DNPWC.
Principle investigator for the project Mr. Dibesh Karmacharya gave overview of the project progress including laboratory analysis; Co- Principle investigator Mr. Kanchan Thapa informed about the Field planning, logistic arrangements and sample collection efforts. Dr. Maheshwor Dhakal, Ecologist, DNPWC discussed about the collaboration efforts and Mr. Netra Sharma , AOTR- NTGP also gave his view on the highlights of the project and collaboration.
In the dissemination, information about first year [2011/12] activities was highlighted:
- Capacity Building
- Field logistics and implementation
- Sample collection, handling and storage methodologies
- Laboratory assay and analysis
As part of capacity building NTGP has worked in the following areas:
- Human capacity building by organizing various trainings by international trainers and international workshops ;
- Training on conservation genetics and data analysis to the Nepali Laboratory associates, conservationists and academician in the USA and Kathmandu;
- Training on field biology to the field staffs at Sauraha, Chitwan;
- International stakeholders’ workshop;
- Laboratory capacity building by introducing advanced molecular technology to the lab and training laboratory associates on them;
- Installation of ABI 310 sequencer, training on application;
- Construction of bioinformatics suite for data analysis and archiving.
Field Logistics and plan implementation was done in consultation with DNPWC, NTNC and the wardens of respective national parks and wildlife conservation areas. In the first phase of sample collection more than 1000 samples have been collected from four focused protected areas, current laboratory analysis verifying over 80% field species identification accuracy of tiger scats. Mark capture recapture sample collection was done in the Chitwan National park. Phase second and third will be conducted in the second year.
All scat samples collected were brought to the Laboratory in Kathmandu; samples were entered into the main database and samples are stored in a secured location. Samples are processed for DNA extraction, species Id, sex Id and microsatellite analysis.
All the stakeholders present in the dissemination showed interest in the project and its achievements so far, and showed their commitment to work together in other species as well.